Antifreeze Protein Improves the Cryopreservation Efficiency of Hosta capitata by Regulating the Genes Involved in the Low-Temperature Tolerance Mechanism

In this study, whether the addition of antifreeze protein (AFP) to a cryopreservative solution (plant vitrification 2 (PVS2)) is more effective in reducing freezing injuries Hosta capitata than PVS2 alone at different cold exposure times (6, 24, and 48 h) investigated. The upregulation C-repeat binding factor 1 (CBF1) dehydrin (DHN1) response low temperature was observed shoots. Shoots treated with distilled water (dH2O) strongly triggered gene expression 6 h after exposure, which higher those expressed PVS2+AFP. However, 24 expressions detected dH2O treatments were similar + AFP. highest PVS2+AFP when time extended h. Similarly, nitric reductase activities (Nia1 Nia2) genes, are responsible for oxide production, also upregulated low-temperature-treated shoots, as CBF1 DHN1 patterns during periods. Based on patterns, shoots likely resist stress, associated cryopreservation efficiency compared alone. This finding suggests that improvement by AFP could be due transcriptional regulation CBF1, DHN1, Nia1, Nia2, might reduce cryopreservation. Thus, potentially used cryoprotectant rare commercially important plant germplasm.